This study was aimed to screen the lignocellulolytic ability of selected fungi under laboratory conditions. In presence of lignin and hemicellulose will reduce the activity of cellulase. Wild type fungal strains which have cellulolytic activity along with lignolytic activity, hemicellulolytic activity, were isolated from different animal dung manure soils collected from Sri Venkateswara Go Samrakshanasaala, Tirupati. All starins were studied by cultural morphological characteristics and microscopic examinations. Seventy fungal strains were isolated and among which ten isolates found to have lingocellulolytic activity. The isolates namely Trichoderma, Aspergillus, Penicillum, Altrernaria, were found to be highly lingocellulolytic compared to the rest. This study showed that the fungal isolates with appreciable hydrolytic zones have good hydrolytic property of lignocelluloses.