The present study focuses on isolation of actinomycetes from the soil in Ulu Kenas forests of Manong, located in the northern part of Perak state in Malaysia and screening for antibacterial activity. All the isolates were screened for their antibacterial activity using Kirby-Bauer (KB) method against Escherichia coli, Pseudomonas aeruginosa, Klebsiella pnemoniae and Staphylococcus aureus. Out of the ten isolated actinomycetes, seven, namely B, D, E, F, H, I and J showed antimicrobial activity against selected bacteria. However, isolates E showed maximum inhibition against P. aeruginosa and the isolates I and J against K. pneumonia with highest scores. The findings of the study may be helpful to the future investigators in identifying alternative and new bioactive secondary metabolites like antibiotics to treat resistant human pathogens.
