Microalgae are photosynthetic prokaryotic and eukaryotic microorganisms. In this research, isolation of microalgae from Maninjau lake West Sumatra was identified by morphological and molecular identification by PCR using primers for 16S rDNA and 18S rDNA prokaryotic and eukaryotic microalgae. The results of this study were obtained 3 isolates of microalgae that can be isolated from Maninjau lake West Sumatra are Scenedesmus (code isolate AUMA-020) with a percent similarity of 95%, Uncultured cyanobacterium (code isolates AUMA-023) with a percent similarity of 80% and Limnothrix (code isolates AUMA-019) has a percent similarity of 98% based on data gene Bank blast results.