The present study is focused on the extraction of intracellular pigment from actinobacteria isolated from the wetland soil sample of Sundarbans, West Bengal. Out of a total 20 isolates, only one actinobacterial isolates showed pigmented growth. Further, the potential isolate was identified by using 16s rRNA sequencing and confirmed as Streptomyces fradiae VITIPMSB. The intracellular pigment was extracted by cell disruption technique using ethyl acetate as the solvent. Partial characterizations of the pigment were carried out using UV-Visible spectrophotometry and FT-IR spectroscopy. The extracted pigment was applied for the preparation of lip balm and assessing its textile dyeing property. In addition, extracted pigment was analyzed for antioxidant and hemolytic activity. The obtained results showed potent antioxidant activity and confers the bio-pigmentas non-toxic to human erythrocytes
