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Isolation and characterization of heavy metal resistant PGPR and their role in enhancement of growth of wheat plant under metal (cadmium) stress condition | Abstract
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Abstract

Isolation and characterization of heavy metal resistant PGPR and their role in enhancement of growth of wheat plant under metal (cadmium) stress condition

Author(s): Chhaya Verma, Pooja Singh, Rajesh Kumar

In present time excessive use of chemical fertilizers in agricultural field causes the environmental hazards and affects the human and animal health. There is an urgent need to reduce the application of chemical fertilizers. Plant growth promoting rhizobacteria (PGPR) is the best alternative of chemical fertilizers, present in root zone of plant and enhanced the plant growth in stress as well as the normal condition. This study was done on heavy metal resistant PGPR, isolated from heavy metal contaminated soil of industrial and agricultural area of Lucknow, Kanpur and Ambedkar Nagar. Out of 27 isolates only 6 were grown on high concentration of cadmium metal. Isolate PBB1 showed 1000 ppm MIC of cadmium and remaining isolates namely PP3, PP2, SNA3, and SNA5 were grown on 800 ppm MIC of cadmium. PGPR screening was done for selection of best PGPR to enhance the growth of wheat plant by production of IAA, ammonia and phosphate solubilization. The pot study was done with 100 ppm cadmium amended soil. When cadmium resistant PGPR were applied on seeds of wheat the growth and germination of plants were enhanced. On comparison of finding result with literature, it may be possible that all the isolates may be fluorescent pseudomonads. The result of pot study showed that the Pseudomonas sp. SNA5 gave the best result in comparison of other isolates.