The aim of the research is isolate and identify the fungal endophytes from the leaves of Crinum asiaticum L. Totally thirteen isolates of fungal endophytes were isolated from the leaf of Crinum asiaticum L. The endophytic fungi such as Acremonium rutilum, Alternaria sp, Alternaria tenuis, Chaetomium globosum, Chrysosporium tropicum, Curvularia sp, Cylindrocarpon candidum, Fusarium solani, Fusarium chlamydosporum, Fusarium sporotrichoides, Fusarium javanium, Humicola sp, Helminthosporium sativum, Nigrospora sphaerica and Verticillium terrestre were isolated. The isolated endophytic fungal characters were conformed.