The present paper deals with In-vitro callus induction and shoot regeneration in Physalis minima (L.). Physalis minima belong to the family Solanaceae, a small herbaceous annual plant grown as weed in the crop field. Ethnobotanical information showed that this plant has tremendous medicinal value for cure out different diseases. Study includes multiple shoot cultures to regenerate whole plant from callus of apical leaf, node and root explants. The present study highlights the importance of plant tissue culture in order to be used for large-scale production of drug at cost affordable levels. This study can be utilized to develop a standard protocol to initiate multiple shoot culture of plant that may provide a good source of pharmacologically active plant constituents. Callus culture were initiated from leaf, shoot tip and root explants on Murashige and Skoog medium supplemented with different concentrations of hormone(s), alone or in combination, for rapid initiation of callus and biomass production. Shoot cultures were regenerated from these three explants and its generation capacity, length and morphology were observed.
