The present paper deals with In-vitro callus induction and shoot regeneration in Ephedra gerardiana from nodal explant. Ephedra gerardiana an evergreen shrub also called as Ma- Haung and in India it is called as Somlata, belongs to family Gnetaceae. It is mostly grow at higher altitudes. Ethnobotanical information showed that this plant has tremendous medicinal value for cure out different diseases. Extracts of Ephedra have been used in traditional Chinese medicine for over five thousand years to treat asthma, nose and lung congestion, hay-fever, malaria and several other ailments. The present study highlights the importance of plant tissue culture in order to be used for large-scale production of drug at cost affordable levels. This study can be utilized to develop a standard protocol to initiate multiple shoot culture of plant that may provide a good source of pharmacologically active plant constituents. Callus culture were initiated from nodal explants on Murashige and Skoog medium (MS medium) supplemented with different concentrations of hormone(s), alone or in combination, for rapid initiation of callus and biomass production. Shoot cultures were regenerated from these three explants and its generation capacity, length and morphology were observed.
