In the present study the protocol for callus induction and regeneration in Boerhaavia diffusa has been developed in culture medium. Young apical leaves, nodal region and roots was used as explants for callus induction on MS medium containing 2-4- D and Kinetin. Callus initiation was first recorded in the lamina of leaf and nodal region. The optimum % of calli was obtained from leaf lamina. The calli in most of the cultures were yellowish white and friable in nature. After callosogenesis the regeneration of shoot takes place.
