Methanolic extracts from different parts of Lantana camara and Mimosa pudica were evaluated for potential antimicrobial activity against medically important pathogenic strains. The present study aimed at evaluating the in vitro antimicrobial activity of methanolic extracts of medicinal plant against Alternaria alternata, Aspergillus niger, Macrophomina phaseolina and Rhizoctonia solani using agar well diffusion technique. The extract showed the highest activity against Aspergillus flavus. Hence, these plants can be used to discover bioactive natural products that may serve as leads in the development of new pharmaceuticals that address not fulfilled therapeutic needs.