Newly, importance has substantially augmented in verdict naturally arising antioxidant to supplant manmade antioxidants, which were circumscribed due to their side effects for instance carcinogenesis. These indicate that the plants are a significant foundation of natural antioxidant, which might be obliging in averting the advancement of innumerable oxidative stresses and Reactive oxygen species (ROS) which have been found to play an imperative role in the commencement and/or advancement of several diseases such as atherosclerosis, inflammatory injury, cancer and cardiovascular disease. Thus, latest studies have scrutinized the potential of plant products as antioxidants against various diseases induced by free radicals. Antioxidant capacity is widely used as a parameter to characterize nutritional health food or plants and their bioactive components. This study is done to evaluate the in vitro antioxidant and free Radical Scavenging potential of the extract of fruits of Citrus limon and Solanum lycopersicum. The plant extract is obtained by cold expression method and the resultant solution is serially diluted to 10, 20, 30, 40, 50 μg/ml. The antioxidant potential was analyzed by hydrogen peroxide scavenging activity seen at 230nm using UV Visible Spectrophotometer. Based on the results obtained in the present study, it is concluded that fruit extracts of Citrus limon and Solanum lycopersicum exhibits high antioxidant activity. The reducing power assay exhibited that reducing capability of the extract were considerably increased with cumulative concentration and were higher compared to the standard ascorbic acid and the antioxidant action was evaluated by scavenging hydrogen peroxide method where the EC50 values of Ascorbic acid, Citrus limon and Solanum lycopersicum were found to be 0.766, 1.418 and 1.3046. Based on the results obtained in the present study, it is concluded that fruit extracts of Citrus limon and Solanum lycopersicum exhibits high antioxidant and free radical scavenging activities.
