In the present investigation anthelminitic activity of the chloroform, methanol and aqueous extracts of the plant spermacoce ocymoides (Burm F) DC (Rubiaceae) were investigated as single extracts and in the different ratio of different extracts on adult Indian earthworm Pheretima posthuma group of Annelida. The chloroform extracts, methanolic extracts and aqueous extracts shown maximum anthelminitic activity in dose dependant manner at 35 mg/ml in ratio of 20 : 40 : 40 respectively which demonstrated paralysis of worms as well as death within 23.5 + 2.6 minute and 44.33 + 5.55 minutes respectively and found to be significant as compared to the standard drug albendazole (30 mg/ml) and piperazine citrate (15 mg/ml). Then suitable formulation was prepared as anthelminitic suspension and the anthelmentic activity of formulated suspension was evaluated on adult Indian earthworm Pheritima posthuma because they belong to same group of Annelida, family Megascolecidae as well as on worm parasites of human beings Ascardia galli (nematodes) which are available in Chicken (Gallus gallus).
