A field study was conducted to study the effect of bio-stimulant AQUASAP manufactured from seaweed Kappaphycus alvarezii on some tropical and subtropical banana varieties such as Karpuravalli (ABB), Rasthali (AAB), Naadu (ABB/AAB) and Ottu (ABB/AAB) by applying 5% through foliar spray at the 3rd, 5th and 7th month of plantation during the year 2012-13. Both quantitative and qualitative data of fruits were examined along with vegetative growth of the plants. The highest yield was found in Naadu with 40.26% over control followed by Karpuravalli, Rasthali and Ottu with 23.38%, 30.03% and 26.61% respectively. Fruits of treated plants also showed less moisture level as compared to control with 42.25%, 33.68%, 49.50% and 30.23% increased protein content in Karpuravalli, Rasthali, Naadu and Ottu respectively. In addition to this 15.75%, 23.55%, 13.95% and 14.26% more carbohydrate content was observed in all the four banana varieties. Therefore Kappaphycus alvarezii seaweed biostimulant can be used in banana for better yield with improved quality.