Since the grain and especially corn is used as energy source in poultry diets, but they can not provide the required energy and due to the beneficial effects of animal fat and plant fat resources, so this experiment used to evaluate the effects of single or combined fats with animal and plant origin in poultry diet on broiler population cecum microflora. Treatments included the first treatment with 4% animal fat of tallow, the second treatment with 4% fat plant of canola oil, the third treatment with 4% plant fat of sunflower oil, the fourth treatment with 2% animal fat of tallow + 2% plant fat of canola oil and the fifth treatment with 2% animal fat of tallow and 2% plant fat of sunflower oil. The results showed that adding plan and animal fat had no statistically significant effect on microbial population of Coliforms bacteria (P>0.05), but addition of fat sources on microbial populations of Escherichia coli was statistically significant effect (P<0.05).