In order to determine the effect of zeolite mineral, bio-fertilizers and different levels of chemical nitrogen fertilizer under limited irrigation water management on peanut yield and its water use efficiency, a field experiment was conducted at Astaneh Ashrafiyeh province located at North of Iran. In 2010 growing season, Factorial split-plot in randomized complete block design with three replications was used. main plot was consisted of water regimes treatments (I1=dry land and I2=12 day Interval Irrigation) and sub factor was consisted of combination treatments of zeolite mineral (Z1= 0 and Z2=7 ton ha-1), bio-fertilizers and different levels of chemical nitrogen fertilizer (N1=0, N2= Azospirillum + Azotobacter, N3= 30 kg N ha-1+ Aazospirillum + Azotobacter, N4= 60 kg N ha-1+ Aazospirillum + Azotobacter and N5= 90 kg N ha-1+ Aazospirillum + Azotobacter) was performed. The results of this investigation showed that all physiological traits (plant growth rate, pod growth rate, partitioning coefficient and during growth period from pod filling) were significantly affected by applying the suggested treatments. The Maximum partitioning coefficient were obtained under 12 days irrigation management (1.14); application of 7 ton ha-1 zeolite (1.143); application 30 kg N ha-1+ Aazospirillum + Azotobacter (1.23)