The present study was designed to evaluate the antibacterial activity of Adathoda vasica against drug resistant uropathogens and to test the efficacy of the plant to stabilize silver nanoparticles [AgNPs]. AgNPs were synthesized using Adathoda vasica leaves. Preliminary formation of nanoparticles was confirmed using UV-Vis spectrum at the absorbance of 420 nm Further it was characterized with FTIR, XRD and SEM analysis. Synthesized AgNPs were then subjected to check the antibacterial activity against a few drug resistant uropathogens like Escherichia coli, Proteus sp., Pseudomonas aeruginosa, S.aureus, Streptococcus sp. and Enterobacter sp. isolated from the patients suffering from urinary tract infection. Also to determine the mechanism of action of the bioactive compound of the plant, docking studies were performed using Schrodinger software. The SEM revealed the sizes of poly-dispersed [50 – 60 nm] nanoparticles in the medium, the silver at nano crystals formation was exhibited by XRD spectrum, and the FTIR analysis represents the biological compounds involved in nanoparticle synthesis. The antibiogram demonstrated that all the uropathogens used in the study were MDR. Antibiotic assay test reveals that the synthesized AgNPs have potent antibacterial activity even in minimum concentration against the uropathogens tested. Docking of the bioactive compound with fimH further demonstrated that the AgNPs exhibited antibacterial activity by preventing bacterial binding. Thus the silver nanoparticles stabilized by Adathoda vasica has shown to possess antibacterial activity and also have the ability to prevent anchorage of bacteria to human cell wall, which may lead to the death of the bacterium.
