The aim of the present study was to assess the effects of ethanolic extract of Piper trioicum on the amylase, lipase and alpha glucosidase activity In vitro. Powder of whole plant of Piper trioicum was extracted in ethanol and the extract was assayed for the measurement of inhibitory effects on activities of enzymes. The extracts rich in bioactive phytochemicals showed inhibitory activity on the amylase, lipase and alpha glucosidase, thus suggesting that extract might be useful in the treatment to limit dietary fat and glucose absorption and the accumulation of fat in adipose tissue. The extracts of Piper trioicum may be safe, natural and cost effective for reducing fat and glucose absorption.