Ascidians are reported to be a rich source of extremely potent bioactive compounds. Phallusia arabica, is a simple ascidian belonging to the family Ascidiidae found in plenty throughout the year along the Tuticorin coast of India. Infrared (IR) spectral study indicates the presence of hydroxyl, carbonyl group and aromatic compounds. GC-MS studies revealed the presence of fourteen chemical constituents n-Decanoic acid, n-Hexadecanoic acid, 7-Nonen- 4,8-dimethyl-2-one, 7-Tridecanone, 1-Iodoundecane, 1,2-diisooctyl ester of Benzene dicarboxylic acid, 2,6,10,15- tetramethylheptadecane, 1-Iodotridecane, 1-Iodododecane, Squalene, 1-Iodo octadecane, 26-Nor-5-cholesten-3a-ol- 25-one, cholestan-3a-ol, 6,22-dien,3,5-dedihydrostigmastan.