The present investigation was aimed to develop an efficient protocol for indirect organogenesis of Solanum trilobatum L. using leaf explants. Development of organs from callus and multiple shoot induction was achieved in the modified Murashige and Skoog (MS) medium enriched with NAA, 2, 4 – D alone and in combinations with various concentrations (0.5, 0.3, 1.0 + 1.0 mg L-1). Morphogenic pale yellow green and greenish brown callus was obtained after 4 weeks of incubation. Multiple shoots were formed from the callus in the MS medium containing BAP combination with KIN at the concentration (1.0 + 0.3mg L-1) after appropriate incubation period. The regenerated shoots were transferred to rooting medium (containing NAA + IBA with concentrations of 0.5 mg L-1 + 0.4 mg L-1) exhibited better roots formation. The complete plantlets were transferred to the poly cups filled with sterile sand and red soil in the ratio of 1:1 and successfully acclimatized in the field.
