Toddy which is considered as tropical alcoholic beverage was procured from Uhukottai area of Tiruvallur (Dist), Tamilnadu, India during early morning from the tender fronds of the inflorescence of Coconut (Cocus nucifera) plant and was employed for the probiotics to analyze their antibacterial potency. Six different bacterial colonies were isolated from the toddy, out of which, a single strain had shown good antagonistic properties against selective human pathogens. The strain was further identified by its 16S rRNA sequence analysis and revealed the microbe is phylogenetically related to the members of genus, Bacillus subtilis sub sp. Further the organism was checked for its intestinal colonization activity by in-vivo analysis using ornamental fish (Black Molly) by microbial plating of intersecting intestine.
