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In vitro rooting of amaryllis (Hippeastrum johnsonii), a bulbous plant, via NAA and 2-iP | Abstract
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Abstract

In vitro rooting of amaryllis (Hippeastrum johnsonii), a bulbous plant, via NAA and 2-iP

Author(s): Sara Zakizadeh*, Behzad Kaviani and Rasoul Onsinejad

This study showed the in vitro rooting of a bulbous plant (Amaryllis) via singular effect of 2-iP and NAA. For rooting of bulblets, different concentrations of 2-iP (0, 14, 16 and 18 mg l-1) and NAA (0, 14, 16 and 18 mg l-1) were used. A well developed root system was achieved in the MS medium containing 16 mg l-1 2-iP and 4 mg l-1 NAA. Rooting is a crucial step for successful micropropagation. The highest root length (2.71 cm), longest root (3.53 cm) and root number (2.77) were obtained in the MS medium supplemented with 4 mg l-1 NAA. The plantlets containing roots were acclimatized (90% survival percentage) by transferring them to plastic pots containing cocopeat, compost and sand.