This study showed the in vitro rooting of a bulbous plant (Amaryllis) via singular effect of 2-iP and NAA. For rooting of bulblets, different concentrations of 2-iP (0, 14, 16 and 18 mg l-1) and NAA (0, 14, 16 and 18 mg l-1) were used. A well developed root system was achieved in the MS medium containing 16 mg l-1 2-iP and 4 mg l-1 NAA. Rooting is a crucial step for successful micropropagation. The highest root length (2.71 cm), longest root (3.53 cm) and root number (2.77) were obtained in the MS medium supplemented with 4 mg l-1 NAA. The plantlets containing roots were acclimatized (90% survival percentage) by transferring them to plastic pots containing cocopeat, compost and sand.