Caralluma adscendens (Roxb.) R.Br. is a traditional medicinal plant which is conserved by in vitro technique of tissues culture using nodal explants. Among various concentrations, best response in terms of multiple shoot formation was observed on Murashige and Skoog medium supplemented with 1.0 mgl-1 Kn. It was produced an average shoot length of 2.98 ± 0.38 cm per explant . 1.0 mgl-1 IAA proved to be an optimal, producing an average root length of 4.98±0.4 cm per explant. The plantlets were successfully established in pots with 73% survival rate which did not show any morphological abnormalities in the field conditions during acclimatization period of 3 months
