The plant Justicia gendarussa was investigated for their antioxidant activity. The extractions were subjected to assay by Reducing power, Nitric oxide scavenging activity, DPPH methods for evaluation of anti oxidant activity. Among these Petroleum ether extract of Justicia gendarussa revealed strongest antioxidant activity. The results primarily suggest the presence of potent oxidant inhibitory principles in the leaf of Justicia gendarussa.