GET THE APP

In vitro Comparative Activity of Chloramphenicol, Virulent Bacteriophages and the combination of both on Salmonella Species | Abstract
Scholars Research Library

Scholars Research Library

A-Z Journals

+44 7389645282

Annals of Experimental Biology

Abstract

In vitro Comparative Activity of Chloramphenicol, Virulent Bacteriophages and the combination of both on Salmonella Species

Author(s): Bala DT, Yakubu SE, Abdullahi IO and Ella EE

The Optical Densities (OD) of both Salmonella species (Ss) and Salmonella Pollurum (Sp) growth after 24 hrs. Infection by phages remained low (SsDW-0.2 ± 0.11; SsKW-0.37 ± 0.15 and SsR-0.19 ± 0.11) while the uninfected control Ss increased to 0.64±0.055. The decrease in OD600 of SsDW, SsR and SsKW were not significantly different from each other at p≤0.05. The difference in OD600 of SpDW (0.25±0.02309 to 0.36±0.03), SpKW (0.2767 ±0.006667 to 0.4667±0.06119) and SpR (0.2467 ±0.02028 to 0.2033±0.08413) were significantly different from each other at p≤0.05 except SpDW and 2SpKW which are not significantly different from each other but lower than the uninfected culture (Sp-0.62±0.02887). The final absorbance of the SsDW (0.2 ±0.11), SsC (0.17 ±0.05) and the combination SsDWC (0.37 ± 0.2) decreased significantly (p≤0.05) compared to that of Ss (0.64 ± 0.055). The activity of DW and DWC were not significantly different from each other. There were significant differences at p≤0.05 between the reductions due to Chloramphenicol (SsC-0.23 ± 0.0033 to 0.17 ± 0.05) and SsKW (0.23 ± 0.015 to 0.37 ± 0.15) compared to SsKWC (0.24 ± 0.01 to 0.14 ± 0.1). Between KW and C, the actions are not significantly different. There is no significant difference between the change in optical densities of SsR (0.25 ± 0.012 to 0.19 ± 0.11) and SsC (0.23 ± 0.0033 to 0.17 ± 0.05) and SsRC (0.23 ± 0.0033 to 0.17 ± 0.13).The optical densities of Sp increased significantly, being the control to 0.62 ± 0.02887 while SpC (0.2467 ± 0.003333 to 0.21 ± 0.03512) and SpDWC (0.24 ± 0 to 0.1267 ± 0.04177) decreased within the incubation period and were not significantly different from each other but significantly different from that of SpDW (0.36 ± 0.03) at p≤0.05. The optical densities of SpKW (0.2767 ± 0.006667 to 0.4667 ± 0.06119), SpC and SpKWC (0.24 ± 0 to 0.15 ± 0.05033) were all significantly different from each other. There was significant decrease (p≤0.05) in the optical densities of Salmonella cultures infected or/and treated with phage SpR (0.2467 ± 0.02028 to 0.2033 ± 0.08413), SpC and SpRC (0.24 ± 0.01155 to 0.68 ± 0.02082) compared to the control Sp but there was a change at the seventh hour where the optical density of SpRC started increasing and even superseded the control at the 17th hour and continued increasing steadily above the control. The OD600 of SpR was not significantly different from that of SpC but both were significantly different from that of SpRC at p≤0.05.