This research is aimed to investigate the cardio protective effect of amylase. Doxorubicn was used to induce toxicity in H9C2 (cardiomyoblast) Cells and were exposed to 6.25 to 100 μg/ml of amylase from Aspergillus niger. After treatment the percentage of cell viability was studied by cytotoxicity assay by direct microscopic observation and by MTT method. The obtained results showed that amylase significantly increased the percentage of cell viability in a concentration dependent manner. Concentration of 50 μg/ml of amylase shows moderate activity whereas 100μg/ml was found to have potent activity. Cell viability at 6.25, 12.5, 25, 50 and 100 μg/ml was recorded as 42.2, 48.5, 50.7, 60.8 and 62.4% respectively by MTT assay. The data revealed that the treatment with amylase induced cell viability
