Callus culture of Adhatoda vasica Nees were established from leaf explant. Different growth regulators greatly influenced the growth of callus cultures. Leaf derived callus grown on Murashige and Skooge (MS) medium fortified with different concentration (1, 1.5, 2,2.5 ppm) of Indole Acetic Acid (IAA), 6-Benzyladenine (BA), Kinetin (Kn), 2, 4- Dichlorophenoxyacetic Acid (2, 4-D). Accumulation of a bioactive pyrroloquinazoline alkaloid, vasicine and vasicinone in callus culture was detected and confirmed by thin layer chromatography. The Rf values of vasicine and vasicinone were found as 0.52 and 0.60
