To investigate the in vitro Antioxidant and Anti-Proliferative Activity of Plectranthus amboinicus leaves extract on MCF-7 cell lines. Qualitative and quantitative analysis of Plectranthus amboinicus using standard methods. DPPH Radical scavenging and cytotoxicity assay carried out by standard methods. Preliminary phytochemical analysis of the methanol, ethanol, chloroform and ethyl acetate extract of Plectranthus amboinicus showed the presence of alkaloids, flavanoids, terpenoids, phenols, saponins, carbohydrates and protein. Quantitative estimation of phenol, steroid and alkaloid was carried out and the phenol was present in higher concentration than other compounds. The antioxidant activity of ethanolic extracts of Plectranthus amboinicus was investigated by in vitro study such as DPPH scavenging assay. This reveals that the ethanol extract posses a good antioxidant activity. Inhibitory concentration (IC50) for the ethanolic extract of Plectranthus amboinicus on MCF-7 cell was found to be at 32.97 determined by MTT assay. The crude ethanolic extracts of Plectranthus amboinicus has antioxidant and antiproliferative activity against MCF-7 cell lines could be due to the presence of n-Hexadecanoic acid and phytol.
