Der Pharmacia Lettre
Abstract
Author(s): Namrata Singh, Neetesh K Jain, Pushpendra Kannojia, Navneet Garud, Anupam K
Pathak and Swaroop C Mehta
Antioxidants play an important role to protect human against infections and degenerative
diseases. The aim of this study is to verify the In-vitro antioxidant properties and to calculate the
total polyphenol contents, total tannins, flavonoid contants of hydroalcohlic extract. The
powdered crude drugs were extracted with hydro-alcoholic solvent (70:30) by double
maceration process. Phytochemical tests of hydro-alcoholic extract reveals the presence of
carbohydrate, alkaloid, flavonoid, steroids, protein, amino-acids and tannins. The antioxidant
activity of hydroalcohlic extract of Calotropis gigantea leaves (HECGL) was investigated using
in-vitro models like DPPH (1,1-Diphenyl-2-Picryl-Hydrazyl) free radical scavenging activity.
Reducing power assay using ascorbic acid, nitric oxide scavenging activity using curcumin
equivalents. At the same time the phenolic content of the extracts was determined using Folin-
Ciocalteau reagent, total tannins and total flavonoids using rutine to evaluate their contribution
to total antioxidant activity. HECGL at 400�Ž�¼g/ml concentration showed maximum DPPH radical
scavenging activity (85.17%) and for nitric oxide scavenging (54.55%) at 100�Ž�¼g/ml
concentration. Reducing power of HACGL was increases with increasing the concentration of
extract. Phenolic contents, expressed as gallic acid equivalents 63.08 Ã?â??Ã?± 4.17 /100 mg of the dried
weight of cg. Tannin 0.52% and Flavonoids 46.97Ã?â??Ã?± 1.95 Ã?ŽÃ?¼g mg-1.It is concluded that, this study
is to verify the antioxidant properties of hydroalcohlic extract, and to define the total polyphenol
contents, flavonoids and tannins in Calotropis gigantea leaves.
