The aim of present study was to estimate the in vitro antioxidant activity of aqueous extract of Cucumis callosus (Rottl.) Cogn (Cucurbitaceae) seeds which is commonly known as “Kachri” in Rajasthan (India) evaluated by using DPPH radical scavenging activity and hydrogen peroxide radical scavenging activity. The antioxidant activity is compared with ascorbic acid as standard. The IC50 values of Cucumis callosus and ascorbic acid were found 37.71 μg/ml and 16.72 μg/ml respectively for the DPPH radical scavenging activity while 94.71 μg/ml and 143.45 μg/ml respectively for hydrogen peroxide radical scavenging activity. Thus, aqueous extract of Cucumis callosus seeds possess potent antioxidant activity in hydrogen peroxide model and may be useful for preparation of neutraceuticals as potent antioxidant to treat various human diseases.
