Aqueous and ethanolic extracts of brussel sprouts were investigated with different in vitro antioxidant activity test including diphenly picryl hydrazyl radical scavenging, lipid peroxidation, reducing power, superoxide anion radical scavenging, and metal chelation. Ethanolic extract showed strong inhibition of DPPH radical scavenging activity than aqueous with IC 50 of 1mg/ml and 1.2 mg/ml. Furthermore, both ethanolic and aqueous showed equipotent activity (IC 50 0.4mg/ml) toward lipid peroxidation of microsomes isolated from rat liver homogenate induced by the FeCl2- H2O2 model. Inhibition of superoxide anion and metal chelation of aqueous and ethanolic extracts showed IC 50 of 0.5 mg/ml, 0.6 mg/ml and 0.2 mg/ml, 0.3 mg/ml respectively. The extracts also showed a strong reducing activity compared to positive control, ethylenediaminetetraacetic acid (EDTA).
