The study was carried out to ascertain the anti bacterial activity of ethanolic extract of Indigofera barberi. The anti-bacterial testing of whole plant of ethanolic extract was evaluated by disc diffusion method using gram positive bacteria like Staphylococus aureus, Bacillus subtilius and gram negative bacteria like Escherichia coli, pseudomonas aeruginosa. Amongst the test extract, the results as per preliminary phytochemical screening presence of flavonoids, steroids, glycosides, carbohydrates and phenols suggested that ethanol extract showed significant antibacterial activity compared with standard gentamycin drug.
