The basic objective of this work was to identify and estimate the active constituents present in the herb Coldenia procumbens Linn. A survey of literature showed that the plant has not been exploited properly and remains a silent drug in herbal medicine. In this work, the coumestan derivative wedelolactone was identified in the methanolic extract of the plant by TLC. The concentration of wedelolactone in the plant extract was found to be 2.2%w/w. An HPLC method was developed for the estimation and the developed HPLC method was validated with respect to LOD, LOQ and linearity. This is the first such report about the plant.
