Ichthyofaunal diversity of Wainganga river in District Bhandara of Maharashtra, India was conducted to assess the fish fauna. The ichthyofaunal of a reservoir basically represents the fish faunal diversity. The present investigation deals with the ichthyofaunal diversity in Wainganga river, Bhandara during the year January 2012 to December 2013. The results of present study reveal the occurrence of total 51 species were identified among those, 22 were of order Cypriniformes, 10 of Siluriformes, 06 of Ophiocephaliformes, 05 of Synbranchiformes, 03 of Perciformes, 02 each of Cyprinodontiformes and Clupeiformes and 01 of Anguilliformes.