A new simple, accurate and precise HPTLC method has been developed for the simultaneous estimation of Atorvastatin Calcium (ATV) and Pioglitazone (PIO) in tablet dosage formulation. In this method, standard and sample solutions of Atorvastatin Calcium and Pioglitazone in tablet dosage form were applied on the stationary phase used was precoated silica gel 60 F under 254. The mobile phase used was a mixture of chloroform: methanol: toluene (6:3:4 v/v). The detection of spot was carried out at 259 nm. The calibration curve was found to be linear between 100 to 400 ng/spot for Atorvastatin Calcium and Pioglitazone. The proposed method can be used to determine the drug content of marketed formulations. The method was validated for precision, accuracy, and reproducibility.
