The present research was carried out to investigate the HPTLC fingerprint analysis and antimicrobial activity of various extracts of leaves of Cassia fistula L. Preliminary phytochemical screening was done by the methods of Treas and Evans and sofowora. The HPTLC analysis were carried out as Harbone and Wagnar et. al. described. The phytochemical screening showed the presence of alkaloids, carbohydrates, glycosides, saponins, triterpenes, tannins, flavonoids, photobatalin and anthraqunies in methanol and aqueous extracts of leaf. The analysis of methanolic extract of leaves by HPTLC confirmed the presence of flavonoids(Peak 4) and alkaloids(Peak 3).The in vitro antimicrobial activity was performed by agar well diffusion method of Perez et. al.,. Of the different extracts methanol, ethanol and aqueous extracts showed significant antimicrobial activity against gram negative bacteria P. aeruginosa and S. aureus also.This study concluded that leaf of C.fistula L. possess significant flavonoids and alkaloids which might be a responsible active principle for the antimicrobial activity.
