Ascidans are a rich source of bioactive secondary metabolites. Phallusia nigra is a simple ascidian belonging to the family Ascidiidae found in plenty throughout the year along the Tuticorin coast of India. The methanolic extract of Phallusia nigra was subjected to HPLC and FTIR spectral analysis to determine the possible bioactive components. The interpretation of the spectrum showed the presence of aliphatic bromo compounds, phenol or tertiary alcohols, carbonyl compound, carboxylic acids, lipids, proteins, alkanes and aromatic compound.
