Detrimental effect of Electromagnetic Field (EMF) on reproductive system has been of critical concern for a long time. The aim of the present study was to evaluate the effect of EMF exposure during developmental period on histophysiology of testis in adulthood. For this purpose the adult male and female Balb/c mice were mated and pregnant mice were divided into control and experimental groups. In the experimental group, the pregnant mice were exposed to 3 mT EMF, 4 hours /day during pregnancy. The control group were treated similar to experimental group but without exposure to EMF. After delivery, both in control and experimental groups the male pups were kept under normal condition till reach adulthood. Then they were sacrificed and their testes were fixed and paraffin sections were stained and examined with light microscope. Microscopy revealed that in the seminiferous tubules from experimental group several intercellular spaces were present and spermatogenic cells appeared to be disrupted. The nuclei of the different spermatogenic cells were dense and hyperchromatic. Evaluation of semen parameters showed that the concentration of sperms and their motility were decreased significantly in comparison to control group. Furthermore, morphological abnormalities were increased significantly in experimental group in comparison to control group. In general, it is concluded that EMF exposure during intrauterine life could affect the semen quality and spermatogenesis in adulthood which may lead in subfertility.
