In India, agriculture forms main occupation of majority of the population. Organic manures such as Farm Yard Manure, green manure in the form of leaf extracts, etc. when incorporated into the soil not only add the nutrients such as nitrogen etc. but the soil is enriched by the fixation of the atmospheric nitrogen. An experiment was carried out with to assess the influence of herbals and biofertilizer on the biochemical parameters of cluster bean (Cyamopsis tetragonoloba L. Var. PNB) . The experiment consisted of six treatments . A study was conducted to assess the effect of Herbals ( Vitex negundo , Curcuma longa, Acorus calamus, Ribes uva-crispa, Acalypha indica) (Ocimum basilicum and Lantana camara) and bio fertilizer ( Rhizobium ) . The biochemical parameters like Protein , Chlorophyll, Carbohydrates and Ascorbic acid were tested on 30th , 45th , 60th and 75th day . The Herbals and biofertilizer were used to observe the change in biochemical parameters. The biochemical parameters of cluster bean was recorded on 30th ,45th ,60th and 75th day . After sowing Chlorophyll ‘a’, ‘b’ and ‘total’ chlorophyll were higher in biofertilizer treatment (T6) on all the days. The maximum protein content was shown on 30th day by T3 on 45th and 60th it was higher in T5 and On 75th it was higher in T6 .The highest carbohydrates was found in T6 on 30 and 45 DAS (79.24 mg / g and 79.95 mg / g). on 60th . The highest carbohydrates content had been observed in T5 and on 75th in T6 . The highest ascorbic acid content had been observed in T6 on 45th and 60th and 75th. Application of biofertilizer with herbals favoured the biochemical’s of cluster bean .