The present study was designed to investigate the effect of fish oil extracted from Sardinella fimbriata against fatty liver diseases in rat experimental models and regenerative property. Ethanol and Methotrexate were administered to eight weeks old animals for 4 weeks to induce Alcoholic fatty liver disease and Non Alcoholic fatty liver disease, respectively. After experimental period, the parameters such as liver weight, food intake, liver histology and liver markers alanine amino transferase, aspartate amino transferase, alkaline phosphatase, direct bilirubin, total bilirubin and gamma- glutamyl transpeptidase were studied. Along with liver markers, lipid profile such as blood glucose, total cholesterol, triglycerides, LDL-cholesterol, VLDL-cholesterol and HDL-cholesterol were also studied. The results of the experimental groups showed elevation of all above mentioned parameters in ethanol and methotrexate treated groups, where as significant reduction in liver markers enzymes with decrease in liver weight were observed in groups which are fed with fish oil along ethanol (Group 5) and MTX (Group 6), suggesting the possibility on the prevention of eventual increase in the release of lipoproteins from the liver even after prolonged intoxication with the development of liver dysfunctions. Histopathological studies showed Steatohepatitis in ethanol (Group 3) and methotrexate (Group 4) intoxicated groups, where as in groups which are fed with fish oil along ethanol (Group 5) and MTX (Group 6) showed ameliorating effects. The liver damage caused by alcohol intoxication was easily reversed; however, it was not in the case of NAFLD in the present study.
