Algae grown on wastewater media are a potential source of low-cost lipids for production of liquid biofuels. This
study was aimed to estimate the effect of Physico-chemical characteristics of normal and sewage water (pH 7.60
and 6.60, EC 15.97and 12.36 μmol, free Co2 1.48 and 0.74, nitrogen 0.90 and 0.50 mg/l, potassium 168.11 and
54.63 mg/l, calcium 249.52 and 112.21mg/l, magnesium 104.91 and 51.19 mg/l, sulphate 57.08 and 28.35 mg/l,
chloride 98.00 and 84.63 mg/l, carbonates 362.18 and 32.64 mg/l and bicarbonates 1138.30 and 253.33mg/l in
sewage and normal water respectively) on Chlorella, Scenedesmus and Botryococus. The highest biomass (4.533 mg
ml-1), chlorophyll (15.56 μg ml-1), lipid (49 %), acid value (0.52mg KOH/g), density (0.885 g/cm3), iodine value (75
mg/g), saponification value (0.125mg KOH/g), viscosity (4.8mm2/sec), myristic acid (9.0%), oleic acid (9.3%),
linolenic acid (20.1%), palmitic acid (35.3%), stearic acid (6.1%) was observed in Scenedesmus than Botryococus
and Chlorella. The properties of algal oil meet all the properties given by American society for testing and
materials (ASTM) D6751, ISO 15607and EN14214- Europe. Hence, it is concluded that algae can be grown better
in sewage water than normal water for their oil and used as a potential feedstock for liquid biofuel production.
