Information on genetic diversity of germplasm collections and determining genetic relations in breed material is prerequisite in plant breeding programs. The following research tries to study 129 genotypes from various locations (10 countries) using 20 pairs of EST-SSR markers. Considering the derived similarity matrix, two genotypes from Shush Region had the highest genetic similarity of 0.894 and two genotypes of 4 and 56 from Shush and Egypt had the lowest genetic similarity of 0.38. Cluster analysis results using Complete classification method grouping the genotypes in three main groups. The close relation between genotypes indicates the marker’s nature, for these markers are designed based on completely preserved and expressible points of genome. Hence, the change and, as a result, variety between genotypes is unlikely.
