The present study evaluates the chemical composition of the essential oils of Juniperus oxycedrus ssp. macrocarpa (Cupressaceae) from El kala region (sub-humid) in Algeria. Leaves essential oils were analyzed by GC/MS. Fifty four compounds were identified. The J. oxycedrus leaves oil was characterized by a high content of Germacrene D (21%), 1,5-Dodecadiene (8,42 %), 2,6,10-Dodecatrien-1-ol, 3,7,11-trimethyl-, (Z,E)- (10,94 %). and 1HNaphtho[ 2,1-b]pyran-3-acetic acid, dodecahydro-3,4a,7,7,10a-pentamethyl-, methyl ester (8,77 %).