Agropyron is one of the most resistant plants to biotic and abiotic stresses. It has important role to produce forage yield in rangelands. Genetic variability based on different markers is the important step in crop improvement. In order to evaluate the genetic variation of different Agropyron species based on morphological, chemical ans karyotipic traits this experiment were carried out in the Agriculture and Natural Resources Research Centre of Kermanshah. The tiller numbers, spike length, spikelet numbers, length of flag leaf, width of flag leaf, plant height, peduncle length, ash percentage, organic matter, fiber, dry matter, fat and crud protein, TF%, Mean Chromosome length, Chromatin length and C.V. were registered. Statistical analysis of data was done by SPSS software. The diploid, tetraploid, hexaploid and octaploid level were observed. According to cluster analysis the tetraploid genotypes were classified into 5 clusters. Based on principal components analysis the length of flag leaf, spike length and plant height, hence among the chemical traits the ash percentage, organic matter and crude protein had highest portion of variance. The genetic parameters such as PCV, GCV, ECV, Hb and Ga for different traits were estimated.
