Genetic relationship among termites collected from various locations was characterized based on 12S rRNA gene using specific primers. Sequence analysis and divergence among the species was assessed. Genbank accession numbers were obtained for the different species. Phylogenetic tree based on Maximum-Likelihood method was drawn on the basis of multiple sequence alignment , which revealed clustering of individuals according to the genera. Among the species, Microtermes obesi and Neotermes koshonensis were distinct from others.