Evolutionary conserved sequence specific gene regulation mechanism “RNAi” is a powerful tool for understanding the molecular pathways, genetic screening of desired genes and silencing the targeted genes. Although, RNAi mechanism is a universal system then present both in many eukaryotes and also prokaryotes with similar complicated mechanism, proteins that involved in the mechanism are shows minor differences. Hence the diversity of the proteins within the mechanism is also allowed to scientist to find phylogenetic relationship within the organisms. But the major importance of the mechanism is comes from its agricultural and pharmacological potential. RNAi guided solutions such as value-added products or patient specific new therapeutics by rational design of the molecular pathways via RNAi mediated gene silencing.
