The genus Hygrophila auriculata contains about 43 species of tendril – bearing climbing herbs distributed in tropical India. The plant was collected at Thottiam village, Tiruchirapalli District, Tamil Nadu. The antimicrobial activity of plant extracts and preliminary phytochemical screening was evaluated with antibiotic susceptible and resistant micro-organisms. It was tested for antimicrobial activity by disc method. The extract of plant showed antimicrobial activity against both gram (+) and gram (-) bacteria. Maximal antibacterial activity was observed against S.epidermidis and maximal antifungal activity was against for Hygrophila auriculata. The ethanolic extract was subjected to GC – MS analysis. Alkaloids, tannins, glycosides, terpenoids, steroids, flavonoids, and saponins are found in the plant extracts.
