The bioactive components of Cissus vitiginea leaves were evaluated by standard protocol using the equipment Perkin-Elmer Gas Chromatography–Mass Spectrometry. The mass spectrum of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. The GC-MS analysis revealed the presence of various compounds like Diethyl Phthalate, Tetradecanoic acid, 2(4H)-Benzofuranone, 5,6,7,7ATetrahydro- 6-Hydroxy-4,4,7A-trimethyl-, (6S-CIS), Neophytadiene, 2-Hexadecen-1-ol,3,7,11,15-Tetramethyl, Hexadecanoic Acid, Dibutyl phthalate, Phytol, Oleic Acid, Octadecanoic acid, Squalene and Bis(2-ethylhexyl) phthalate in the methanolic extract of Cissus vitiginea. These findings support the traditional use of Cissus vitiginea in various disorders.