Silver is an active area of academic and more importantly in nanotechnology for application research. This source of production of nanoparticles can be exploited further commercially. In the present study, we have reported biological synthesis of silver nanoparticles by using cell free filtrate of Helminthosporium tetramera. Synthesis of Silver nanoparticles was carried out using fungal cell free filtrate and 1mMaqueous solution of silver nitrate (Ag No3). The synthesis of silver nanoparticles was investigated by UV-Vis spectroscopy. Absorbance was recorded from 300-600 nm and strong absorbance peak of AgNPs was observed at 400 nm. The obtained Silver nanoparticles were characterized by Transmission electron microscopy (TEM). The synthesized silver nanoparticles were polydisperse spherical in shape and size in the range between 17-33 nm. The antibacterial activity of AgNPs have been evaluated against the Bacillus subtilis and Staphylococcus sp. AgNPs were found to have significant antibacterial activity, thus product could be used as an effective antibacterial agent.
