Corn is one of the major crops, which is cultivation in different regions of Iran. Corn grains during harvest and post-harvest infected by several fungi seed borne, including toxinproducer fungi which the fungi are major share in digestive disease such as cancer in humans and animals. To determine mycoflora corn grains harvested was sample from maize cultivated regions in Golestan province such as Gorgan, Kordkuy, and Bandar gaz, Gonbad, Minoodasht and Kalale. To isolate fungi from maize seeds was used freeze Blotter method. In study, means of incidences Fusarium spp. were the highest (35.2%), followed by Aspergillus spp. (2.9%), Penicillium spp. (1.1%), Rhizopus spp. (2.3%), Mucor spp. (1.4%), and Alternaria spp. (0.2%). Among Fusarium species studied (51.8% of the total isolations), F. Proliferatum(90.1, 42.6%) had the highest percentages of frequency and the highest incidence in Gorgan. 3.5% of all samples were infected by A. flavus species which its of frequency and incidence were 2 and 40.2 percent, respectively and the highest level of infection was belonged to Bandare gaz seeds studied. Penicillium Species were isolated from most samples investigated which the highest incidence (2%) was in seeds studied in Kalale. In this study, among fungi species isolated, F. Proliferatum and A. flavus were identified as important as fungi seed borne and toxin-producer fungi in corn seeds in Golestan province.
