Anti oxidants are emerging as prophylactic and therapeutic agents for various diseases The present study deals with an antioxidant potential of methanolic extract of Ecbolium viride (Forssk).Alston roots. The antioxidant potential of methanolic extract of Ecbolium viride (Forssk).Alston roots was investigated by employing three different established in vitro methods, such as DPPH radical scavenging activity, Nitric oxide radical scavenging activity and reducing power assay. Total flavonoid content was also determined by colorimetric method. The extract was found to be rich in flavonoid content (78±4.8mg quercetin equivalent/g dry weight of extract) and the data obtained in these three methods (78.25%, 69.79% and 0.2756(absorbance) at 100μg concentration respectively) comparable to standard. Results obtained in the present study reveal methanolic extract of Ecbolium viride (Forssk).Alston posses significant antioxidant activity.